Gene Pulser Xcell Total System from Bio-Rad

Product Specs
ItemGene Pulser Xcell Total System
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number1652660
Brand/TrademarkBio-Rad Laboratories, Life Science Group
QuantityEA
ApplicationsElectroporate both eukaryotic and prokaryotic cells
Dimensions(WxDxH) 31 x 30 x 14 cm (12.2 x 11.8 x 5.51 inches)
Voltage10 to 3000 V
Cell TypeEukaryotic and Prokaryotic cells
Weight6.6 kg
MethodElectroporation

Specifications/Features

Pulse Voltage:
10–3,000 V

Capacitance:
10–500 V, 25–3,275 degrees F in 25 degrees F increments
500–3,000 V, 10, 25, 50 degrees F

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Sequencing-based functional assays for classification of BRCA2 variants in mouse ESCsCell Reports MethodsNovember 20, 2023Kajal Biswas, Alexander Y. Mitrophanov, Sounak Sahu, Teresa Sullivan, Eileen Southon, Darryl Nousome, Susan Reid, Sakshi Narula, Julia Smolen, Trisha Sengupta, et al.PR-A7231 Micropulser electroporator,Bio-Rad,Cat# 1652100 Gene Pulser Xcell Electroporation Systems,Bio-Rad,Cat# 1652660

(2) High throughput clone screening on overexpressed hERG1 and Kv1.3 potassium channels using ion channel reader (ICR) label free technologyHeliyonOctober 1, 2023Alberto Montalbano, Cesare Sala, Ginevra Chioccioli Altadonna, Andrea Becchetti, Annarosa ArcangeliCuvettes (Biorad 0.2 cm gap). After being incubated on ice for 10 min cells have been electroporated in a Gene Pulser Xcell Total System (Biorad). The magnitude of applied exponential wave pulse was 320 V, 900 μF. Electroporated cells have

(3) Lactobacillus plantarum surface-displayed FomA (Fusobacterium nucleatum) protein generally stimulates protective immune responses in miceFrontiers in MicrobiologySeptember 20, 2023Xiaoyu Zhang, Huijie Xiao, Huaiyu Zhang, Yang Jiangovernight. The next day, we picked single colonies for identification. Gene Pulser Xcell Total System #1652660 (Bio-Rad Laboratories, Inc., USA). This FomA gene fragment was then inserted into the pSIP409-pgsA'

(4) An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragmentsSTAR ProtocolsSeptember 1, 2023Xiaoqian Zeng, Shuliu Wang, Mindong Liang, Weishan Wang, Yue Jiang, Fei Xu, Leshi Liu, Hao Yan, Yaojun Tong, Lixin Zhang, et al.into E. coli (Gibco, Catalog# 18297010) using the Gene Pulser Xcell Total System (Bio-Rad, Catalog# 1652660).Note: The home-made electrocompetent E. coli cells can also be prepared according to a previous protocol

(5) The effect of histone deacetylase inhibitors on the efficiency of the CRISPR/Cas9 systemBiochemistry and Biophysics ReportsSeptember 1, 2023Ymer Björnson, Codey Y. Huang, Jaedyn L. Rollins, Guadalupe Castañeda, Navneet Kaur, Emiko Yamamoto, Jennifer M. JohnstonNucleofection Electroporation was performed using either a BioRad GenePulser Xcell Electroporation (#1652660) or a Lonza Amaxa 4D Nucleofector (Lonza #AAF-1002B) with the corresponding X unit (Lonza #AAF-1002X).

(6) Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variantsNature communicationsJuly 26, 2023de Melo GD, Perraud V, Alvarez F, Vieites-Prado A, Kim S, Kergoat L, Coleon A, Trüeb BS, Tichit M, Piazza A, et al.Mirus Bio™ Ingenio™ Electroporation Solution (MIR50114) using the Gene Pulser Xcell Electroporation System (1652660, Biorad) with a pulse of 270 V and 950 μF. The cells were then transferred to a T75 culture flask with

(7) Optogenetic modulation of guanine nucleotide exchange factors of Ras superfamily proteins directly controls cell shape and movementFrontiers in Cell and Developmental BiologyJuly 10, 2023Dhiman Sankar Pal, Yiyan Lin, Huiwang Zhan, Tatsat Banerjee, Jonathan Kuhn, Stephenie Providence, Peter N. DevreotesElectroporation Cuvettes, 0.1 cm gap (BIO-RAD #1652089). Gene Pulser Xcell Electroporation System (BIO-RAD #1652660). 0.1 cm-gap cuvette (BIO-RAD, 1652089). Lipofectamine 3000 Transfection Reagent. 10 cm cell culture

(8) Excessive transcription-replication conflicts are a vulnerability of BRCA1-mutant cancersNucleic acids researchMay 22, 2023Patel PS, Algouneh A, Krishnan R, Reynolds JJ, Nixon KCJ, Hao J, Lee J, Feng Y, Fozil C, Stanic M, et al.Electroporation was performed at 1.2 kV, 25 μF and 200 Ω using Gene Pulser Xcell™ Total System (Bio-Rad cat: 1652660), then immediately 1 ml of SOC media (Invitrogen cat: 15544034) was added to the cuvette. Bacteria were

(9) CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signalingNucleic acids researchMay 22, 2023Matsumoto M, Modliszewski JL, Shinozaki K, Maezawa R, Perez VM, Ishikawa Y, Suzuki R, McKnight KL, Masaki T, Hirai-Yuki A, et al.T7 RiboMAX™ Express Large Scale RNA Production System (Promega) as per manufacturer's protocol. Transfection of viral RNA (5 μg) was performed in a Gene Pulser Xcell Total System (Bio-Rad) or using TransIT-mRNA Transfection Kit (Mirus) for subgenomic HAV-Luc RNA as described (3,12). Lentivirus

(10) The exocyst complex is an essential component of the mammalian constitutive secretory pathwayJournal of Cell BiologyMay 1, 2023Conceição Pereira, Danièle Stalder, Georgina S.F. Anderson, Amber S. Shun-Shion, Jack Houghton, Robin Antrobus, Michael A. Chapman, Daniel J. Fazakerley, David C. Gershlickaccordingly. To recover the cell secretion phenotype, day 5 transient KO cells were electroporated (Gene Pulser Xcell Total System; Bio-Rad) with a guide-resistant plasmid encoding the corresponding protein of interest. In brief, 1

(11) Wnt/β-catenin signaling induces axial elasticity patterns of Hydra extracellular matrixiScienceApril 21, 2023Mariam Veschgini, Ryo Suzuki, Svenja Kling, Hendrik O. Petersen, Bruno Gideon Bergheim, Wasim Abuillan, Philipp Linke, Stefan Kaufmann, Manfred Burghammer, Ulrike Engel, et al.Microscope,RRID:SCR_015572 "Gene Pulser® Cuvette, 0.4 cm electrode gap",Bio-Rad,Cat# 1652088 GenePulser XcellTM,Bio-Rad,Cat# 1652660

(12) Non-muscle myosin II drives critical steps of nematocyst morphogenesisiScienceMarch 17, 2023Niharika Garg, Urška Knez Štibler, Björn Eismann, Moritz Mercker, Bruno Gideon Bergheim, Anna Linn, Patrizia Tuchscherer, Ulrike Engel, Stefan Redl, Anna Marciniak-Czochra, et al.Microscope,RRID:SCR_015572 "Gene Pulser® Cuvette, 0.4 cm electrode gap",Bio-Rad,Cat# 1652088 GenePulser XcellTM,Bio-Rad,Cat# 1652660

(13) Construction and application of a heterogeneous quality control library for the Xpert MTB/RIF assay in tuberculosis diagnosisFrontiers in Cellular and Infection MicrobiologyMarch 17, 2023Zehao Guan, Xuefei Han, Weigang Huang, Xueliang Wang, Hualiang Wang, Yun Fanand 10% glycerol); the competent cells in a 1-mm electroporation cuvette were shocked using a pulser (Gene Pulser Xcell Total System; Bio-Rad, Hercules, CA, USA) at 2100 V, 25 μF, and 200 Ω; and 50 μg/ml kanamycin was used for selection

(14) Cyclic AMP is a critical mediator of intrinsic drug resistance and fatty acid metabolism in M. tuberculosiseLifeFebruary 22, 2023Andrew I Wong, Tiago Beites, Kyle A Planck, Rachael A Fieweger, Kathryn A Eckartt, Shuqi Li, Nicholas C Poulton, Brian C VanderVen, Kyu Y Rhee, Dirk Schnappinger, et al.added. Electroporation was performed using the Gene Pulser X cell electroporation system (Bio-Rad #1652660) set at 2500 V, 700 Ω, and 25 μF. Bacteria were recovered in 7H9 for 24 hr. After the recovery incubation,

(15) Genetic Manipulation of Corynebacterium mastitidis to Better Understand the Ocular MicrobiomeInvestigative Opthalmology & Visual ScienceFebruary 17, 2023Yannis Rigas, Benjamin R. Treat, Jackie Shane, Robert M. Q. Shanks, Anthony J. St. Leger1-mm cuvette containing our electrocompetent bacteria. This mixture was then electroporated using a Gene Pulser Xcell Total System (Bio-Rad, Hercules, CA, USA) at 1.8 kV, 25 µF, and 200 ohms. After electroporation, the bacteria were

(16) N-Phenylpyridine-3-Carboxamide and 6-Acetyl-1H-Indazole Inhibit the RNA Replication Step of the Dengue Virus Life CycleAntimicrobial Agents and ChemotherapyFebruary 16, 2023Aïssatou Aïcha Sow, Felix Pahmeier, Yann Ayotte, Anaïs Anton, Clément Mazeaud, Tania Charpentier, Léna Angelo, Simon Woo, Berati Cerikan, Darryl Falzarano, et al.cells were then transferred to an electroporation cuvette (0.4 cm gap width; Bio-Rad) and pulsed with a Gene Pulser Xcell Total System (Bio-Rad) at 975 μF and 270 V. Cells were then seeded in a 15 cm petri dish. One day after electroporation,

(17) De novo biosynthesis of berberine and halogenated benzylisoquinoline alkaloids in Saccharomyces cerevisiaeCommunications ChemistryFebruary 9, 2023Jianing Han, Sijin Litransformed at equimolar ratios into yeast by electroporation, at 540 V, 25 μF and infinite resistance with a Gene Pulser Xcell Total System electroporator (Bio-Rad). Cells were then recovered immediately in 1 mL YPD at 30 °C for 2 h and spread

(18) Global Lipidome Profiling Revealed Multifaceted Role of Lipid Species in Hepatitis C Virus Replication, Assembly, and Host Antiviral ResponseVirusesFebruary 7, 2023Khursheed Ul Islam, Saleem Anwar, Ayyub A. Patel, Mohammed Tarek Mirdad, Mahmoud Tarek Mirdad, Md Iqbal Azmi, Tanveer Ahmad, Zeeshan Fatima, Jawed IqbalThe J6/JFH-1 (genotype 2a) RNA was in vitro transcribed and electroporated into Huh7.5 cells using Gene Pulser Xcell Total system (BioRad). Cell free virus contained in the cell culture supernatant was also propagated in Huh7.5 cells

(19) Bacterial crystalline cellulose secretion via a supramolecular BcsHD scaffoldScience AdvancesDecember 16, 2022Wiem Abidi, Marion Decossas, Lucía Torres-Sánchez, Lucie Puygrenier, Sylvie Létoffé, Jean-Marc Ghigo, Petya V. Krastevato a prechilled 1-mm electroporation cuvette (Eurogentec). Electroporation was carried out using the Gene Pulser Xcell Total System (Bio-Rad) with a 10.2-ms exponential decay pulse of 2.4 kV, with 400-ohm resistance and 25-μF capacitance.

(20) Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genome-wide RNA Interference ScreeningGenomics, Proteomics & BioinformaticsDecember 1, 2022Khaista Rahman, Muhammad Jamal, Xi Chen, Wei Zhou, Bin Yang, Yanyan Zou, Weize Xu, Yingying Lei, Chengchao Wu, Xiaojian Cao, et al.into the competent cells of M. tuberculosis H37Ra using Gene Pulser Xcell Total System (Catalog No. 1652660, BIO-RAD, Hercules, CA) with default settings (2.5 kV, 25 µF, and 1000 Ω resistance). The bacterial

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